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What You Need to Know
Explain what it means
to say that DNA replication is semi-conservative.
Explain the
significance of complementary base pairing in the
conservation of the base sequence of DNA.
Explain what is meant
by DNA replication occurring in a 5' to 3'
direction.
Explain the process of
DNA replication in eukaryotes including the role of
enzymes (helicase, DNA polymerase III, RNA primase,
DNA polymerase I, and DNA ligase), single stranded
binding proteins, Okazaki fragments and
deoxynucleoside triphosphates.
What is an Origin of
Replication? Contrast the number of origins in
prokaryotic cells to the number in eukaryotic cells.
Why is replication
different on the two strands of the DNA helix?
Contrast replication
on the leading strand with replication on the
lagging strand.
What are primers and
why are they needed during replication?
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