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Draw the
basic structure of a DNA nucleotide. |
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Draw the
molecular structure of deoxyribose and
number the carbon atoms. |
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Identify
the four bases of DNA based on the numbers
of rings (purines vs. pyrimidines) and the
number of hydrogen bonds it can form. |
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Outline
how the DNA nucleotides are linked together
by covalent bonds into a single strand
(i.e., to which carbon atoms of the
deoxyribose are the phosphates and bases
attached?) |
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Know
which bases are capable of complimentary
pairing. |
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How many
bases are there per complete turn of the
helix? |
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Outline
the structure of nucleosomes, referring to
the correct number of histone proteins.
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Describe
what is meant by “anti-parallel.” |
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Identify
the 5’ and 3’ ends of a DNA molecule. |
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Describe
the structure of the DNA found inside
mitochondria and chloroplasts, and explain
how this is evidence for the endosymbiotic
theory. |
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What are
restriction enzymes?
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Where are
they found naturally? What is their role in
nature? |
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What is a
restriction site?
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Define
palindrome. |
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How are
restriction enzymes named? |
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Contrast
sticky vs. blunt ends. |
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Why are
restriction enzymes used in DNA
electrophoresis? |
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What is
electrophoresis? For what purposes is it
used? |
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Based on
what properties are fragments separated? |
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What are
the functions of the buffer and loading dye? |
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Describe
the process of DNA fingerprinting.
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Read a
DNA fingerprint to determine relatedness or
forensic guilt.
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Be able
to read a pipette to determine its range of
volume measurement. |
