ChiSquare (X2) Goodness of Fit
Chisquare Goodness of Fit is a statistical test commonly used to compare observed data with data we would expect to obtain. Were the deviations (differences between observed and expected) the result of chance, or were they due to other factors?
Just like other statistical tests, the ChiSquare Goodness of Fit tests two hypotheses:
Null Hypothesis:
"There is not a significant difference between what was observed and what was expected; any difference between observed and expected is likely due to chance and sampling error." For example:

Alternative Hypothesis:
"There is a significant difference between what was observed and what was expected; the differences between observed and expected is likely not due to chance or sampling error." For example:

How to Calculate a ChiSquare Goodness of Fit
 The first step in the calculation of an X2 value is to determine the expected numbers. In genetics, you'd use a Punnett square to determine the theoretical expected values.
 Then, use the formula for each observed and expected category: (OE)2 / E
 The results are added together to get a final X2 value.
 The calculated X2 value is than compared to the “critical value X2” found in an X2 distribution table.
 The X2 distribution table represents a theoretical curve of expected results. The expected results are based on DEGREES OF FREEDOM. Degrees of Freedom = number of categories – 1.
 The X2 distribution table is organized by the Level of Significance. The level of significance is the maximum tolerable probability of accepting a false null hypothesis. We use 0.05.
 If our calculated value is lower than the 0.05 level of significance, we can accept our null hypothesis and conclude that there is NO significant difference between the observed and expected values.
 If our calculated value is higher than the 0.05 level of significance, we can reject our null hypothesis and conclude that there IS a significant difference between the observed and expected values.
Performing a ChiSquare test in Excel 2016
 Enter your observed and expected values in columns.
 Click the box in which you want the Chi Square value to be placed
 Select Insert Function from the Formulas tab
 Search for Chi Square test and select the CHISQ.TEST from the menu
 Hit OK
 Select all of your observed (actual) results for the Actual_range and all of your expected results for the Expected_range.
 Hit OK
 The resulting value is the P value for the ChiSquare test. If you don't want it to be in scientific notation, you can change the format of the number by selecting "number" instead of "scientific."
 If the pvalue you get is less than 0.05, reject the null hypothesis and conclude that there is a significant difference between the observed and expected values. Likewise, if the pvalue is more than 0.05, accept the null hypothesis and conclude that there is no significance difference between the observed and expected.
Performing a ChiSquare test with the TI83/84
 Press [2nd MATRIX]
 Select [EDIT  > 1:A]
 Copy the data by typing in each number and then pressing ENTER
 Now press STAT. Under the TESTS submenu, scroll down and select C:X2 TEST. Press ENTER.
 Move the cursor down to DRAW and press ENTER.
 If the pvalue you get is less than 0.05, reject the null hypothesis and conclude that there is a significant difference between the observed and expected values. Likewise, if the pvalue is more than 0.05, accept the null hypothesis and conclude that there is no significance difference between the observed and expected.